Hello,

I'm doing PCR and gel electrophoresis on tetranucleotide microsatellite repeat of a species of diploid salamander. The gel results yield three bands in two sample (attached; red arrows), and I'm confused about how should I interpret this. Other samples (not shown) also display three bands. I only expect from one to two bands in diploid organism. There are a lot of non-specifics, but the microsatellite alleles are highly pronounced.

Thank you

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