Minh T. Le

5 Questions 8 Answers 0 Followers

Questions related from Minh T. Le

What could cause this asymmetrical smear on gel electrophoresis?

Hello, I'm doing PCR and gel electrophoresis on tetranucleotide microsatellite marker. Recently, I have often encountered strange, asymmetrical smears on the gel (images attached). I suspect this...

06 June 2019 655 6 View

How do I interpret a triallelic pattern in a diploid organism's microsatellite?

Hello, I'm doing PCR and gel electrophoresis on tetranucleotide microsatellite repeat of a species of diploid salamander. The gel results yield three bands in two sample (attached; red arrows),...

06 June 2019 1,951 2 View

What cause smearing in DNA agarose gel electrophoresis?

Hello, I have been having trouble with smearing PCR product of a type of marker in my gel lately (picture is attached). I'm working with a triploid organism, so three bands can be observed, but...

05 May 2019 3,955 5 View

Is it safe to de-dilute DNA sample by evaporating the solvent?

Hello. I accidentally add to much solvent (Qiagen AE buffer) into my DNA sample and the DNA concentration is too low. Is it safe for me to incubate my DNA tube with the cap slightly ajar to get...

05 May 2019 297 2 View

What does an extra, unclear band coupled with a clear band indicates in gel electrophoresis?

Hello, I'm using PCR to assess the differences between the banding patterns of a microsatellite in different salamander species. In my resulting gel, there's extra unclear bands underneath the...

12 December 2018 6,454 7 View