Detecting brain cancer with convolutional neural network(CNN): How to choose hyperparameters (i.e.number of filters, filter shape, max-pooling shape)?

Hi everyone, I've decided to detect brain cancer form an image library, so I wanna use the CNN algorithm to extract features from the library and train my neural network but still I wanna know how...

19 August 2020 3,734 3 View

What is an appropriate time interval for correlation of annual SPEI time series?

Hi! I'm comparing two 50-year SPEI (Standardized Precipitation Evapotranspiration Index) time series created from model and observed data (1950-1999) and averaged over a large area. Both time...

14 October 2019 1,459 3 View

Is it possible to calculate the average pore width using DFT-model?

I have tried to calculate/estimate the average pore size using DFT-model asuming slit formed pores. I am using the ASAP 2020 instrument but the instrument only gives me the total volume of the...

30 June 2019 8,406 3 View

Are there known factors/parameters affecting the sialylation/desialylation of recombinant glycoproteins produced in cell cultures?

Can a human recombinant glycoprotein produced in mammalian cell culture lose part of its sialic acid residues due to changing conditions in the culture or the purification of the protein? Any...

17 May 2019 9,954 3 View

Why can't I detect p-Akt bands in my western blots?

I am looking for phospho-Akt (serine 473) in rat heart tissue samples using western blots. I have done trouble shooting for weeks but still nothing. At first we thought it was our antibodies that...

06 June 2016 4,922 5 View

What is the best tissue homogenization method for reverse lectin ELISA?

Our initial plan is to use streptavidin or neutravidin coated 96-well plates (15115, Thermo Scientific) and biotinylated lectins to isolate glycoproteins from complex samples then probe for...

10 June 2015 7,426 2 View

How much tissue of a mouse heart is sufficient for protein extraction?

Does anyone know how much tissue is needed of a mouse heart to obtain sufficient protein for western blotting? We would like to cut a mouse heart longitudinally and use one half for blots and the...

24 March 2015 7,681 7 View

Can anyone recommend a method for Mining Gene Expression Omnibus (GEO) for the expression of 1 gene?

17 October 2014 3,008 5 View

Does anyone have experience in TNF-alpha detection: ELISA vs Western Blots?

Good day For my MSc I wanted to determine the levels of TNF-a released by H9c2 cells in response to treatment with Doxorubicin. I treated every day for 5 days, so after each day I collected the...

06 August 2014 2,818 18 View

No title

My institution is considering buying it for our in-house library, but we're hesitant because there are no reviews on Amazon yet. We're particularly interested in how well mathematical and...

19 March 2014 4,184 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Mixed ANOVA - help?

I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...

27 February 2021 9,626 4 View

How to Interpret Partial Eta Squared Numbers For a Repeated Measures Anova?

I have obtained Partial Eta Squared Numbers through Two-Way Mixed Anova Analysis. My results for different constructs are: .105, .135, .038, .068, .061 respectively. I wanted to know, whether...

24 February 2021 7,122 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Interpretation of Goodman - Kruskal Gamma squared?

Dear colleages, Eta squared is dominantly used in reflecting the explaining power the same manner as a squared partial correlation coefficient (R2p ) from multiple linear regression: as the...

22 February 2021 7,801 3 View

Interpretation of a fluorescence Emission spectrum ?

Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...

20 February 2021 1,291 3 View

In CDA analysis we have three stages, viz., description , interpretation, and reproduction. Why have all CDA studies neglected the third stage?

In CDA analysis we have three stages, viz., description , interpretation, and reproduction. Why have all CDA studies neglected the third stage?

18 February 2021 6,483 3 View