What should be the method for the immobilization of lipase into cellulose paper?
What should be the coating onto the cellulose paper so that the lipase can be immobilized ?
Use any cross linker which provide CHO groups.
1)gluteraldehyde or formaldehyde coupling
2) Homo-bifunctional imidoesters like N-hydroxysuccinimide esters, dimethyl adipimidate (DMA), dimethyl suberimidate (DMS) and dimethyl pimelimidate (DMP)
3) Heterobifunctional crosslinkers like Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate)
Also Read paper
A zero-step functionalization on paper-based biosensing platform for covalent biomolecule immobilization
Regards
Use any cross linker which provide CHO groups.
1)gluteraldehyde or formaldehyde coupling
2) Homo-bifunctional imidoesters like N-hydroxysuccinimide esters, dimethyl adipimidate (DMA), dimethyl suberimidate (DMS) and dimethyl pimelimidate (DMP)
3) Heterobifunctional crosslinkers like Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate)
Also Read paper
A zero-step functionalization on paper-based biosensing platform for covalent biomolecule immobilization
Regards
Hi Akshita, do you know what functional groups are in your cellulose paper?
Your cellulose has -OH groups, but what about aldehydes (-CHO), acids (-COOH) or primary amines (-NH2)?
This is important information because they require different chemistries to conjugate proteins.
Hi Dear Akshita Mehta
On the cellulose paper only -OH functionality is present. So you can easily reacted with epichlorohydrin to create functional epoxy (or oxiran) groups on the cellulose under alkaline conditions. This reaction also provides some degree of cross-linkage on the cellulose. The available epoxy groups can be used for covalent coupling of lipase enzyme via -NH2, or -SH groups of the enzyme at pH between 8.8-9.0.
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