Hello :)
I want to run various analyses on human whole-stool samples frozen at -80 °C, including metabolomics and shotgun sequencing of the stool microbial community. Since I cannot introduce another freeze-thaw cycle, the samples must always stay frozen. I need to homogenize the samples because neither metabolites nor microbial cells are evenly distributed throughout a stool sample. Does anybody know a (cheap) device for this purpose? Many devices can only accommodate much smaller samples, unfortunately. Are there large and very robust plastic bags in which one could place the stool in case one were to crush them mechanically (e.g., with a hammer)?
Thank you
Friederike
You can use liquid nitrogen to freeze them and crush with mortar and pestle. The sample can be homogenized and can be used for further analysis including molecular works like isolation of DNA/RNA for NGS or any other biochemical assays.
You can use liquid nitrogen to freeze them and crush with mortar and pestle. The sample can be homogenized and can be used for further analysis including molecular works like isolation of DNA/RNA for NGS or any other biochemical assays.
You can use liquid nitrogen to freeze them and crush with mortar and pestle. The sample can be homogenized and can be used for further analysis including molecular works like isolation of DNA/RNA for NGS or any other biochemical assays.
Its consistency is basically like a rock, yes. Crushing it with mortar and pestle is impossible because it's too hard. Plus, it should not defrost.
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