Help regarding free-floating immunohistochemistry on mouse spleen?

Hello fellow researchers I am trying to stain cryostat-sectioned spleen tissue, using free-floating chromogenic immunohistochemistry. Unfortunately, I'm not able to detect any staining (MHC class...

13 November 2020 6,884 3 View

How much plant powder do I need for different concentration of two extracts (Aqueous and Ethanolic extract)?

Supposed I need three different concentration for two plant extracts (namely: Aqueous and Ethanolic extract), starting at 50mg/ml, then 100mg/ml then 150mg/ml. In what particular weight of plant...

31 March 2019 2,775 5 View

Does anyone have any experience running ERIC-PCR?

11 May 2016 526 39 View

Any papers that have specific gases and its composition in carbonization of coconut shell?

carbonization, coconut shell, gases, carbonizer

24 February 2016 2,412 3 View

Dissertation query, Q - method inquiry. currently running a pilot study and am falling short on the analysis. All info welcome?

Hi, I'm an Undergraduate student at the University of Chichester and I'm currently writing my dissertation. I have all my statements and my question, i have knowledge of exel and spss software...

04 January 2016 3,364 4 View

What factors affect the electrode polarization for lithium-ion batteries?

Surface area, particle size? What other possible factors reduces/increases Electrode Polarization? And how does Electrode Polarization affect affect rate-capability and battery performance?

08 December 2015 2,303 6 View

Who will assist me in developing a new theoretical concept on our oceans' formation - ?

Hello and thank you for welcoming me here. I am thrilled to announce that I seem to have developed an in itself seemingly "water-proof" raw theory on the formation of our (and any) planet's...

01 September 2015 2,035 6 View

Did someone try to ablate metallic cerium?

I want to ablate metallic cerium but i'm concerned about the inflammable nature of cerium. Any suggestions? Thank you!

24 August 2014 1,285 2 View

How do I perform an ELISA validation?

I am performing a (partial) validation of commercially available ELISA kit. I am using the kit on a previously untested matrix, so I wish to validate the assays Accuracy, intra-assay Precision and...

02 April 2014 8,905 3 View

Does anyone have information (citation) or experience about cyclic olefin copolymers (COC)?

I'm interested in preparation, chemistry (stability, solubility, water content), and characteristics (optical (fluorescent), thermal, electrical....), particularly biocompatibility. Processing...

15 June 2013 6,288 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View