I tried to isolate proteins from microalgae (Chlorella and Chalmaydomonas), using trizol method. After, isolation I could not see any pellets/precipitate of protein.
Can anyone suggest me how can I get protein precipitate?
Would you try Protein precipitation Berges et al.(1993).
I thought the following papers may be of some help to you.
1.AMINO ACID COMPOSITION AND NUTRITIVE
VALUE OF THE ALGA SPZRULZNA MAXIMA
By G. CLfiMENT, C. GIDDEY and R. MENZI
2.An evaluation of methods for extraction and quantification of protein from
marine macro- and microalgae
Elisabete Barbarino1,2 & Sergio O. Louren¸
It depends on which kind of proteins (e.g., soluble, insoluble, membrane proteins) from which cellular compartment you want to isolate. The method of how you disrupt the cells is also important.
With respect to Chlamydomonas reinhardtii there is some information and there are also several cross references on page 260 / Chapter 8 / Volume 1 of the second edition of the Chlamydomonas Sourcebook by Harris EH, Stern DB, Witman GB (2009), Academic Press, San Diego, CA.
Because protein purification is much more complex and error-prone than one might think, you also may want to get more comprehensive information about the critical parameters. For it,see (for example):
Protein Purification: Principles, High Resolution Methods, and Applications 3rd Edition, Jan Christer Janson, Wiley; 2011
Thank you very much. But, unfortunately, I don't have free access of this book......if you have can you send me?
Protein Purification: Principles, High Resolution Methods, and Applications 3rd Edition, Jan Christer Janson, Wiley; 2011
This paper may be helpful
Extraction, fractionation and functional properties of proteins from the microalgae Chlorella vulgaris by Alina-Violeta Ursua, b, Alain Marcatic, Thierry Saydd, Véronique Sante-Lhoutellierd, Gholamreza Djelvehc, , , Philippe Michauda