What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

Sample is in a powdered form - how do I prepare for DLS?

I have this question from @Anurag Nath posted on an existing thread: https://www.researchgate.net/post/What_are_some_effective_sample_preparation_techniques_for_DLS_study To avoid confusing the...

06 January 2021 4,856 2 View

Does anyone have an insight into the behavioural response of fishes to moving wind turbine blades as seen through the air-water interface?

I am interested to find out if wind turbines located close to rivers or lakes, or perhaps even in marine locations, are a potential problem for fish. This seems to be a very under-researched area...

04 January 2021 3,468 2 View

Why is my TiO2 sol milky?

@Krunal Dholiya has sent me this question which I post below so he can get the benefit of many experts. Dear, I am preparing a thin film using TiO2 sol but i m fasing problem, with time it is...

23 November 2020 1,188 2 View

What do you think makes an effective classroom-instruction?

class management ;interaction between teachers and students;

16 November 2020 5,301 4 View

Zeta potential and concentration?

I have a question from @Pratima Adhikari that I will post on the open forum so all experts can contribute. Hi Alan, I hope you are fine and healthy. I have a question for you. I am currently...

22 October 2020 3,377 3 View

Which software can be used to prepare nexus file for phylogenetics analysis in Mr Bayes?

which software can be used to prepare nexus file for phylogenetics analysis in Mr Bayes? which format is best interleaved or sequential formats? Can you please explain?

22 February 2020 4,878 3 View

Possibility of inculcating INTELLIGENT MANAGEMENT SYSTEM to Higher education system?

The impact of IMS(INTELLIGENT MANAGEMENT SYSTEM) in the industry as well as economy in multiple countries at multilevels had been creating immense impact in the way the whole system was developed...

03 December 2019 4,284 3 View

What can be the possibility of inculcating behavioral finance to the existing studies conducted?

What are the possibilities of incorporating BEHAVIOURAL FINANCE to the existing stream of studies that already gave a clear base to the subject for Phd? What are the possibilities of looking at...

03 December 2019 5,884 11 View

What should be the basic text/document to refer upon for researches(especially social sciences)?

The fact that better knowledge on basic research comes out of understanding the existing techniques and methods is the key for a fruitful research. 1.Which should be considered as the primary...

23 November 2019 3,725 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View