Hot alcoholic extraction can extract glycans, and cold acetone and alkali can extract proteins. Actually both are intermilgled very tightly hence, both can not be extracted in one. Similarly, separate extraction should be done for carbohydrate as well as proteins

I read some papers in which the researchers used Guanidine HCL to resolve the proteins and then dialyzed the extraction into UREA, following that DEAE columan was used to elute the PGs. But I need a protocol with detailed procedures and comments.

Isolation of proteoglycans is a multistep complex process. I isolated the PG of palmar fascia. If you want, I will send you the materials and methods section of my dissertation. unfortunately it is Polish.