Hello Everyone,
Am trying to develop an enzyme hydrolysis protocol using bromelain. The specific activity of the bromelain enzyme is 4.5 U/mg protein (provided by the supplier).
My task is to hydrolyze a fish sample using an enzyme sample ratio of 1% (1 g of enzyme powder added to 100 g of fish).
However, I observed from literature that enzyme sample ratio is frequently expressed as U/100 g of sample.
Hence, how do I convert the 1% enzyme-sample ratio to U/100 g of fish?
Hi Adeseye,
Please check the following link. Hopefully it will help.
http://www.physiologyweb.com/calculators/units_per_volume_solution_concentration_calculator.html
Thanks Kaveh. I think I figured it out. Just need to determine the enzyme activity of the bromelain and express the value in U/mg of enzyme solids. From this, I can calculate the activity (U) that correspond to 1g, and then express this value in terms of U/100g of sample.
I agree, you must determine the activity (U) per mass of powdered enzyme.
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