I want to titrate my rabbit anti-human FPR1 antibody and would like to perform the titration on cells that I know strongly express FPR1 on their surface.
You may able to prime the cells with fMLP or LPS or TNF-alfa. If you are just going to test antibody for in vitro titration experiment incubate about 0.1 to 10 nanomolar fMLP for FPR receptor expression on isolated neutrophils for about 1h. Measure antibody binding with and without fMLP stimulation.
Thanks Mahendra. After reading some older papers, I think I will choose 50ng/mL PMA for neutrophil stimulation for 5 min at 37C. PMA seems to increase surface expression of the FPR.