How do I do an electrophoresis for a large Bacmid (about 140 kb)?

More Ebrahim Kord's questions See All

Please, could someone lend a hand in acquiring an article related to radiation physics ?

Dear ResearchGate community, I'm currently in search of an article for my research. I've been unable to locate it thus far and would be immensely grateful if anyone could kindly assist me in...

08 May 2024 7,958 0 View

How to calculate the apparent viscosity in porous media(the flow that has shear and elongation flow)?

According to the "chauveteau 1981" article, the apparent viscosity decreases at low shear rate and then increases after a critical shear rate. He didn't mention how to calculate the apparent...

16 April 2024 4,861 4 View

How to make the pressure dimensionless when the Re number is changing?

Hello everyone I'm simulating some problem (fluid flow in a wavy channel) with increasing the Re number and i want to check pressure difference in it. I need the pressure difference to be...

04 March 2024 2,824 4 View

How to resect the superior mesenteric ganglion in mice?

I am trying to resect the CG-SMG in mice. Although I can locate the right area as in the attached photo, I could not identify the ganglion. Any help would be greatly appreciated....

28 February 2024 4,672 0 View

How can I make sure the topic of my master's thesis is good enough and will be valued later?

I'm currently studying geotechnical engineering. I want to choose a topic and don't have any idea where to start. any suggestion would be appreciated.

30 January 2024 3,897 3 View

What are the reliable markers for detecting colorectal cancer using IHC in murine cryo-sections?

I am seeking advice in identifying reliable and specific markers for accurately detecting tumour regions in colorectal cancer mouse cryosections? Any recommendations or insights would be greatly...

03 January 2024 6,040 2 View

What percentage of error is acceptable for two graphs to be in-phase?

I have two graphs of pressure and velocity at a point over the time and they need to be in-phase . There is a small value of phase difference between these graphs. What percentage of this value is...

18 December 2023 3,427 4 View

Transverse Joint Configuration Reference for widening of flexible pavements?

I'm currently facing difficulty locating a reference for the transverse joint configuration for widening of a flexible pavement. From memory, I recall using a range of 300mm to 500mm for...

12 December 2023 1,352 4 View

Why doesn't the velocity fluctuate in a 2D wavy channel in high mesh number but it does in low mesh number?

hello everyone. i'm working on a fluid flow into a wavy channel and i wrote a time-varying equation code of pressure for inlet boundary condition of pressure in openfoam and set the...

05 December 2023 9,107 2 View

What boundary condition should i set for inlet and outlet of velocity to determine it based on pressure gradient?

Hello everyone. I'm working on a 2D wavy channel and simulating a fluid flow. I wrote a time_varying pressure equation code at the inlet boundary condition of P file in openfoam and set the zero...

27 November 2023 2,158 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

I encountered a situation where the 16s PCR amplification worked perfectly with the DNA from the same sample, but using the same sample Don't working?

I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...

24 July 2024 9,688 0 View

How to extract RNA from neurons?

Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...

23 July 2024 6,783 1 View

Help. How can I fix my T7 reaction problems?

I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...

16 July 2024 4,604 0 View

Qiagen circulating nucleic acid kit poor yield?

Hi, Attempted to extract cfDNA from a 4 mL plasma sample, with poor yield. Went through the manual in the section regarding poor yield, and feel that this does not apply. Some potential...

14 July 2024 7,475 1 View

How much 1X TAE buffer should I use in preparing 1.2% agarose gel?

Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...

12 July 2024 2,058 5 View

How to improve my plaque assay for influenza virus?

I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...

11 July 2024 5,669 5 View

Can I use DNA a ladder for RNA samples in agarose electrophoresis? Or It is preferable to use cDNA amplified from RT-qPCR for the same samples?

I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...

09 July 2024 4,857 2 View