Hello, I am trying to perform gap-junction blocking experiments with Heptanol, which is not miscible with water. I have a 1M solution of Heptanol dissolved in 100% ethanol that I am adding to my perfusate to yield a (heptanol) concentration of 1.5mM. However, I am concerned that this is yielding the same result as simply adding raw heptanol to my perfusate (i.e. that the heptanol separates into its own phase). I should say that I have seen the method I am currently using in papers trying to achieve the same gap junction blockade.
Am I doing this right? If someone with experience of using heptanol for electrophysiology or calcium imaging could help me out, that would be much appreciated.
Thanks.
Hi Chris,
You should be fine, no worries. Actually, at this concentration, you should even be able to dissolve heptanol directly into your perfusate. But I do agree that it is better to make first a stock solution in ethanol.
Best, Norbert
The only think you should check is that your 0.15% final ethanol has not effect by itself on your preparation (but it should be fine).
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