When to include covariates?

Hi it's me again! Coming to you with a question about including covariates in a model. So I'm running a mixed effect model in my longitudinal imaging data. I have 4 possible covariates to...

11 February 2021 1,404 1 View

SPSS ANOVA with 2 groups, 3 time points and hundreds of dependent variables?

Hello everyone! I need to run ANOVAs for multiple variables that take place at 3 time points. So I have 2 groups (controls and patients), 3 time points for each measure, and then a plethora of...

22 November 2020 8,032 5 View

Is confocal microscopy not applicable without fluorescence?

Whenever I read about confocal microscopy, it is used in conjunction with fluorescence. Wide-field microscopy also has out-of-focus information blurring the in-focus image. It seems that the...

14 October 2020 6,134 5 View

Fatal error using gmx trjcat to demultiplex trajectories from REMD sims: what is "write_trxframe can not write unknown"?

Hello, I've used GROMACS to perform a short REMD simulation (200 ps) in order to test whether my choice of temperature range allowed sufficient exchanges between replicas. The REMD simulations...

02 September 2020 9,304 3 View

How can I measure learners' overall level of "investment" (Norton,2015) quantitatively?

I would like to investigate students' level of investment (not motivation) in an English class. are there any questionnaires I could use for this?

24 March 2020 6,940 3 View

The appropriate rank of a nothotaxon involving subspecies?

21 December 2019 8,905 3 View

Does anyone know of a cell line (from C57BL/6 mice) that can induce lymphomas (preferentially NHL) when introduced in SCID mice?

Similar to BJAB (human) or A20 (balb/c) but a C57Bl/6 one

07 May 2018 3,920 2 View

Articles about femicide and media coverage?

Does anybody has articles about femicide and media coverage? Thanks Ariana

31 January 2016 840 3 View

Do you include "zero growth" tumors in the statistical analysis of flank assay using Nude mice?

I am injecting prostate cancer cell lines PC3 and LNCaP (1 million and 2 million respectively) into the flanks of nude/J mice. In some of the mice (from both control and treatment group) the...

13 January 2016 2,566 10 View

What is the best way to deliver a miRNA to a tumor with dense extracellular matrix, say pancreatic or kidney?

We see deregulated microRNA in every cancer and in blood.

06 September 2013 120 3 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

Sequence of an unknown Oligonucleotide ?

Hi All, I do have a single-stranded oligonucleotide of 100 - 150 bases synthesized and the sequence is not known. How would I proceed to know the sequence .

04 February 2021 5,614 3 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

How to extract a single stranded DNA from a double stranded DNA sample isolated from stem borer(rice affecting insect) ?

How to extract a single-stranded DNA from a double-stranded DNA sample isolated from stem borer(rice affecting insect)? As I need to amplify ss Dna using a COX-1 marker having primer sequence as...

22 January 2021 3,424 3 View

Can we use the single-elimination tournament method as the basis of an experiment design?

I want to conduct an experiment in the field of Human-Computer Interaction, where I test the perception of users about which image is better using different camera lenses. In the experiment, I...

18 January 2021 779 2 View

How to get the rupture force vs loading rate plots through SMD simulations from gromacs?

Hi I have force vs time graph and Pulling com positions vs time graphs and got from SMD simulations in gromacs the pulling velocity I am using is constant at 0.01 and the force constant was 1000...

16 January 2021 9,080 5 View