I am currently working on DNA methylation and I needed to determine 1ug of the extracted DNA sample to run my MSAP analysis. I'm stuck on how to get the this done and I need help
You need to get your extracted DNA (Before bisulfite conversion) and nanodrop it to get a ug/ul concentration. From there you will know how many ul you will need to get Xug/ul
Hi Michael,
As mentioned before, you need to measure your DNA concentration (either with Nanodrop or with Qubit, which is more sensitive). Then, when you have your concentration, you need to calculate which volume of your sample contains 1ug of DNA. So to say, if you have 20ul of DNA with concentration of 0.25 ug/ul, you will have 1ug of DNA in 4ul of your sample. Then you usually need to add water to your sample to bring it up to the input volume of your kit/reaction etc. In general, the bisulfites conversion kits are usually flexible and allow usage of relatively wide range of input concentrations. In other words, if the concentration of your initial sample is too low, you can still give it a try and check with qPCR afterwards.
Good luck!
Michail
I am really glad about the solutions provided to my question on the DNA. I must say that I have been able to do the work through the insight given here. Thank you all
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