How can I design an experiment capable of demonstrating if my cells can grow without a substrate?

Dear Researchers, I am incredibly confused about the in vitro colony forming assay. I was asked to perform such assay to assess my cell models capability to grow without attachment to a solid...

10 June 2020 6,230 3 View

How to perform NGS sequencing for SARS-COV-2?

My group would like to perform sequencing through Next-Generation Sequencing (NGS) in SARS-COV-2 isolates. However, although many groups have used this technique recently in their research, the...

28 April 2020 4,504 3 View

How can I distinguish between mouse dendritic cells and monocytes?

I would like to identify mouse DC and distinguish them from monocytes in whole blood and tumor tissue mouse samples, by flow cytometry. May I do this using a panel with CD11c, CD11b, LY6C, F4/80,...

21 November 2018 2,113 3 View

Do you know how to release bacteria after adhesion to epithelial cells?

11 January 2018 7,377 6 View

Can anyone help me with primer design for biomarker research?

I'm trying to determine a strategy to study the expression of certain parasites' biomarkers in different conditions. Firstly, I do not know if the best approach would be to start with common PCR...

25 March 2014 3,863 3 View

Experimental or quasi-experimental?

I am currently reading one paper which randomly assigned students of four classes for experimental and control conditions. However this randomization process was within class and not in a whole,...

01 January 1970 1,538 3 View

How can I peel off PDMS from an acrylic mold with a micropattern?

I'm trying to make micropatterns on acrylic to use it as a mold for PDMS. The first time I tried this, PDMS got stuck to the micropattern area of acrylic, even though I made a silanization to the...

01 January 1970 809 19 View

Hello fellow researcher .. What is the meaning of your name?

I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.

03 March 2021 5,266 4 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

How to get rid of somebody pretending to be the author of a publication?

One of the publication I co-authored has the author's name in abbreviation. Then, somebody whose nothing to do with that paper acquired it as her work. To whom in this RG shall I ask for a hand,...

27 February 2021 4,206 4 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View

How can I find a Raman spectrum wavenumber corresponding to what compound?

I have several Raman spectra with very distinct peaks at certain wavenumbers, such as 277, 359, 466, 583, 623, 686, and 795cm-1. How can I identify them to what compound(s)? The RRUFF and others...

24 February 2021 363 3 View