Hello,
I am currently trying a purification method for virus like particles (VLP). The principle behind the method is that the purifying compound causes the produced VLP to aggregate which upon microfiltration remains in the retentate while other compounds like protein goes off, however, I need an experiment that will prove that the method is working outside the reverse HLPC which I can use to assay for purity. Every idea is welcomed, thanks.
Hope this article can be helpful for you,
A comparison of methods for purification and concentration of norovirus GII-4 capsid virus-like particles. Huhti et al 2010.
https://link.springer.com/content/pdf/10.1007%2Fs00705-010-0768-z.pdf
Dear Chisom,
Hope these links will be helpful for you.
https://fenix.tecnico.ulisboa.pt/downloadFile/563345090413156/Public%20version%20-%20Master%20thesis%20Pedro%20Aranha.pdf
https://www.microbiologyresearch.org/docserver/fulltext/jgv/75/8/JV0750081849.pdf?expires=1549032707&id=id&accname=guest&checksum=2BAB7F1C46FAE3CF750FE72D1621DE86
Best Regards.
Marcellin.
Hello,
I am not sure if you are still looking for a solution but Size exchange chromatography can observe aggregation and contamination in viral particles. typically Sephacryl S-300 or S-500 can work well. Superose 6b can also separate viral particles. Size exclusion is a bit of a pain but it is very gentle so it is suitable for a study into the effects of your method.
You should be able to show the viral particle starting material is more aggregated after the addition of your compound (a shorter retention time), the removal of contaminants after filtration and (i am assuming this is the intent) reversal of aggregation to give a pure viral particle solution.
GE healthcare provide the SEC media I was referring to. Hope that helps!
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