How do I concentrate exosomes obtained by SEC (size exclusion chromatography)?

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How to avoid high viscosity of cell pellet in order to resuspend and make them pass easier from a needle?

Hello everybody, i noticed that after centrifugation it is impossible to resuspend the formed cell pellet (3x10^6 cells more or less) due to the high viscosity. I DO NOT want to lyse the cells....

25 October 2020 3,308 2 View

Which inclusion criteria are you going to use?

Both short and tall children

20 February 2017 6,862 1 View

How is it possible to polarize the monocytes in TAMs?

Hello, i decided to ask this question, because i am quite confused regard this topic. I've red that in order to obtain TAMs from freshly isolated monocytes, i should first stimulate with MCSF and...

14 March 2016 1,847 5 View

Is possible to differentiate freshly isolated monocyte to M2d subtype?

Hello, i would like to ask you how it is possible to differentiate freshly isolated monocyte to M2d subtype (TAMs). I studied about M2a,b,c differentiation but it is not clear to me how is it...

14 January 2016 2,951 2 View

Could please someone explain why a western blot can become in this way?

Hello, i load this TGX gel based on the volume and not on the quantity (Ι mean same volume for all the samples). I know that the protein quantity increases in the final samples, but i cannot...

01 December 2015 4,839 7 View

Does anyone have experience with Rhodiola rosea vegetative propagation?

I am interested in vegetatively propagating approximately 6-month-old open-pollinated Rhodiola rosea plants, and I was wondering if anyone knows of an effective way to do so with relatively fast...

21 December 2014 1,899 5 View

Does anyone have a protocol about protoplast isolation from fresh berries and grapes?

This is a project regarding anthocyanin localisation in plant cell vacuoles, and for that reason we need a protocol about protoplast isolation and extraction so we can then take a look at it in...

16 October 2014 2,745 2 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View