Hello everybody,
i noticed that after centrifugation it is impossible to resuspend the formed cell pellet (3x10^6 cells more or less) due to the high viscosity.
I DO NOT want to lyse the cells. I just want to separate them and destroy the clusters they normally form in order to inject them using a needle, without damage them.
It's a suspension cell line.
Thank you very much
I am not a cell researcher, and only saw this question browsing randomly, so please factor that into this answer. It may be that the centrifugal force is too high, packing and compressing cells beyond their ability to later separate. Perhaps reducing the speed while increasing the time if centrifugation would still provide the needed pellet density but prevent cell cohesion. Alternatively, if the centrifuge parameters cannot be changed, then the pellet could be separated mechanically. Introducing ultrasonic vibration might disrupt the cohesion without breaking down the cell membranes. Initially a general crush, followed by in vitro ultrasonic frequency sweep..?
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