Meropi Plousiou

5 Questions 6 Answers 0 Followers

Questions related from Meropi Plousiou

How to avoid high viscosity of cell pellet in order to resuspend and make them pass easier from a needle?

Hello everybody, i noticed that after centrifugation it is impossible to resuspend the formed cell pellet (3x10^6 cells more or less) due to the high viscosity. I DO NOT want to lyse the cells....

26 October 2020 3,323 2 View

How is it possible to polarize the monocytes in TAMs?

Hello, i decided to ask this question, because i am quite confused regard this topic. I've red that in order to obtain TAMs from freshly isolated monocytes, i should first stimulate with MCSF and...

15 March 2016 1,871 5 View

Is possible to differentiate freshly isolated monocyte to M2d subtype?

Hello, i would like to ask you how it is possible to differentiate freshly isolated monocyte to M2d subtype (TAMs). I studied about M2a,b,c differentiation but it is not clear to me how is it...

15 January 2016 2,967 2 View

Could please someone explain why a western blot can become in this way?

Hello, i load this TGX gel based on the volume and not on the quantity (Ι mean same volume for all the samples). I know that the protein quantity increases in the final samples, but i cannot...

02 December 2015 4,862 7 View

How do I concentrate exosomes obtained by SEC (size exclusion chromatography)?

i performed SEC in order to isolate the exosomes by the cell supernatant. I obtained all the fractions (40 fractions of 500μl each) and i would like to ask you which is the best way to extract and...

23 November 2015 646 3 View