Can I use the result from a pilot study to decide the minimum sample size ?

Can I use the result from a pilot study to decide the minimum sample size required to compare the means using independent sample t-test ? Or any other way that can help me to determine the...

29 January 2021 7,677 6 View

Yield of Plasmid is low in Maxipreparation of plasmid for 200ml? What might be the reasons for it?

Hi, I am working with a plasmid which is known to have very low copy number and when I am isolating it by midiprep alkaline lysis method, I am getting good amount of plasmid i.e., 16.56ng/ul....

11 December 2020 1,369 3 View

How to fix the fungus for SEM imaging?

I want to perform the SEM of candida sp. and i want to know that the fixation for the bacteria and fungus are different or same?

10 December 2020 6,169 3 View

I need Coronary Angiogram Images for Coronary Artery Disease detection?

I need Coronary Angiogram Images(CAI) publically available dataset. Coronary Artery Disease (CAD) is a condition of the heart due to atherosclerosis. Atherosclerosis is the narrowing of arteries...

07 December 2020 6,470 3 View

Can satellite spectrum be reused indoors for D2D communication?

Cognitive radios can help to share the spectrum among different networks, both satellite and terrestrial. Is it possible that we use the satellite spectrum for D2D/IoT communication in an urban...

28 August 2020 8,228 7 View

What is the minimum sample size that is acceptable to conduct a survey?

I'm doing a study to evaluate the adherence rate of clinicians to a clinical practice guideline using clinical vignettes (an online survey). The study only includes physicians who are dealing with...

31 July 2020 5,804 4 View

To study antioxidant profile which would be better to analyse ,Serum or Tissue from mice?

I am working on toxicity induced by drugs on invivo model(mice) and few compounds that have antioxidant properties that would be used to reduce this toxicity. The organs I would be looking for...

22 March 2020 4,155 6 View

Mobile Edge Computing SImulator?

So for starters, how can we create a web-based API for creating a web-enabled simulator for Mobile Edge Computing

11 March 2020 9,793 3 View

how to purify and quantitate nanoparticles once synthesized for use in invivo studies?

I am working on nanoparticles and its biological applications . My question is once nanoparticles are synthesized by what methods can they be purified to eliminate contaminants,impurities present...

27 February 2020 2,054 5 View

Difference in type of semiconductor ?

Why there is difference between Halleffect and sensing measurement in the type of semiconductor? Example the first declare p type semiconductor while the second declare n type?

23 February 2020 3,217 6 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Why RFLP Digestion looks in this way?

01 February 2021 9,576 3 View

What is the maximum storage time for samples used in soil enzyme assays?

01 February 2021 4,616 3 View