How do I calculate the ICV infusion dose for drugs published only with subcutaneous infusion?

More André Barreto Vianna's questions See All

Which methods are most commonly used to measure (assess) neuroinflammation in the CNS?

And neurodegeneration in mice.

04 May 2014 2,115 19 View

Does neuroinflammation and neurodegeneration induced by continuous infusion (intracerebroventricular) of LPS occur in all CNS neurons?

Does neuroinflammation and neurodegeneration induced by continuous infusion (intracerebroventricular) of LPS occur in all CNS neurons or only in specific regions? The literature is rich in...

04 May 2014 4,731 2 View

Anyone know a good model to induce hypothalamic inflammation?

I am looking for models to induce hypothalamic inflammation in mice. The most common model is through high-fat diet administration, but I'm looking for something related to TNF-alpha or other...

02 March 2014 5,095 4 View

Does anybody have experience with confocal imaging of mice brain?

Does anybody have a good protocol for perfusion fixation of the brain of mice, as well as, some tips about the protocol of immuno? I want to obtain images of the hypothalamus.

02 March 2014 6,358 10 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

How to determine tannin content in fodder pellets?

22 July 2024 8,163 3 View

When I am trying to distill triethyl amine while drying it over calcium hydride. How do I design the setup to let hydrogen escape without losing NEt3?

I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...

21 July 2024 7,284 4 View

Jordi S Silvestre Popular answer

The better way to 'deduce' a ICV is from knowing the effective concetration (e.g. IC50 or EC50) to the the target for this drug.

Jordi S Silvestre

Seyyed Mohammad Taghi Mansouri

I'm agree with Mr. Jodi S Silvestre

Yameli Fausto

Ademas tienes que tener en cuenta las dosis toxicas