Hi Sara,

The chitinase assay is very simple using chitin as substrate. You can follow these 2 publications of our group. We are working on chitinase.

http://www.nature.com/articles/srep14395

http://www.ncbi.nlm.nih.gov/pubmed/25895958

If you give a brief description of your problem in assay we can try to suggest more particularly.

Thank you,

Best luck with your research.

Thanks a lot  Mr. Ashok. please read this article. i  face to some question:according to this article what is blank and  what is it percent of DNS reagent made ?  how to prepare 1% of DNS from this?

which is better for this work ;this article or yours ?and which do you recommend

"thank you so much"

Please refer to this article. 

http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2007.02117.x/pdf

we used this method in our work with minor modification.

Best luck with your research.

Ashok

thank you so much Mr. Ashok

These articles will be helpful for your study.

Please see

https://www.researchgate.net/publication/311668087_Improvement_of_chitinase_Pachi_with_nematicidal_activities_by_random_mutagenesis

https://www.researchgate.net/publication/282153514_Enhanced_nematicidal_potential_of_the_chitinase_pachi_from_Pseudomonas_aeruginosa_in_association_with_Cry21Aa

https://www.researchgate.net/publication/275278845_Effects_of_domains_modification_on_the_catalytic_potential_of_chitinase_from_Pseudomonas_aeruginosa

Thanks regards

Article Improvement of chitinase Pachi with nematicidal activities b...

Article Enhanced nematicidal potential of the chitinase pachi from P...

Article Effects of domains modification on the catalytic potential o...

thanks a lot dear

can you please tell how can i stabilize chitinase? bcz my enzyme lost its half activity within few days after incubation

Article Enhanced nematicidal potential of the chitinase pachi from P...