Reducing capacity
Experimental groups Absorbance % Reducing capacity
Mean ±SEM
Control 0.056 ±0.003 --
Control +Std 25µg 0.124±0.003 140
Control + SMEE 10µg 0.113±0.005 100
Control + SMEE 20µg 0.150±0.005 120
If the absorbance (OD) was measured at 700nm in spectrophotometer and increase in absorbance of the reaction mixture indicate increase in reducing capacity and the % reducing power calculated using the formula:
% increase in absorbance= Control (OD)- Test OD)/Control X100
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If the absorbance (OD) was measured at 700nm in spectrophotometer and increase in absorbance of the reaction mixture indicate increase in reducing capacity and the % reducing power calculated using the formula: % increase in absorbance: Control - Test
Control
Questions: How do you arrive at the answers using this formula.