Reducing capacity

Experimental groups           Absorbance                   %  Reducing capacity

                                            Mean ±SEM

Control                                0.056 ±0.003                            --

Control +Std   25µg             0.124±0.003                           140

Control + SMEE 10µg          0.113±0.005                            100

Control + SMEE  20µg         0.150±0.005                           120

If the absorbance (OD) was measured at 700nm in spectrophotometer and increase in absorbance of the reaction mixture indicate increase in reducing capacity and the % reducing power calculated using the formula:

     % increase in absorbance=   Control (OD)- Test OD)/Control X100

              -

                                           

If the absorbance (OD) was measured at 700nm in spectrophotometer and increase in absorbance of the reaction mixture  indicate increase in reducing capacity and the % reducing power  calculated using the formula:   % increase in absorbance:  Control - Test

                                                                           Control

Questions: How do you arrive at the answers using this formula.

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