During phage dialysis,its been written somewhere that dialysis process helps to remove CSCL particles from phages,but how its possible as phage size is smaller than cscl , so if cscl moves out from membrane then phage too can move outside?
Adam B Shapiro
Cesium chloride (CsCl) is a salt with molecular weight 168. Phage are very large macromolecular complexes. CsCl is easily removed by dialysis. Phage will not pass through dialysis membrane (typical molecular weight cutoff is 10,000 Da).
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Nagarjun Kasaraneni
I agree Adam, for phage sample dialysis, we use like 100 kDa, it work good.
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