What is DST purifcation in PCR Primer designing?

31 January 2022 1 5K Report

I read about DST purification in primer designing manuals but its signifcance and procedure is not cleary explained in any literterature or source.

Manuele Martinelli

Dear Naveen Chaudhary

i'm not sure but i suppose that DST refer to "desalted", which means salt free oligos which is the Basic oligo purification protocool.

https://eurofinsgenomics.com/en/products/dnarna-synthesis/purification-and-yields.aspx#tabs-1814

https://www.biosearchtech.com/support/resources/oligo-purification#:~:text=%20Purification%20options%20%201%20Salt-free%20%28%E2%80%9Cdesalted%E2%80%9D%29.%20All,eliminate%20any%20fluorescent%20contamination%20remaining%20from...%20More%20

best regards

Manuele

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