I would like to know if I it is possible detect H2O2 in Transmission electron microscopy through DAB and osmium tetroxide reaction. Doe anyone know if it is a possible to detect hydrogen peroxide by stain it in bloco and then observe on TEM?
Hello,
maybe you can have a look to the following article:
Article Reactive oxygen species and ascorbate–glutathione interplay ...
Dear Nicolar Barois,
Thank you for your message. In fact those authors cited the reference of
https://www.scopus.com/record/display.uri?eid=2-s2.0-67649506240&origin=inward&featureToggles=FEATURE_NEW_DOC_DETAILS_EXPORT:1
DOI10.1094/MPMI-22-7-0868
"using the cerium chloride technique which leads to the formation of per- hydroxide precipitates in presence of H2O2, was performed to localize H2O2"
"Leaf pieces were immersed in this incu- bating solution under vacuum to allow a rapid penetration of CeCl3 into plant cells. After four washes in 50 mM MOPS buffer, cells or plant tissues were fixed in a mixture of 100 mM sodium cacodylate buffer (pH 7.2) containing 1.25% (vol/vol) glutaraldehyde and 1.25% (wt/vol) paraformaldehyde for 1 h at room temperature. After fixation, samples were washed sev-eral times in the same buffer over a period of 30 min"
It was a great! Thank you for shed light on this topic.
Cheers,
Joao
Hi Joao Paulo
I used cerium peroxide technique to evidence H2O2 deposits in ultratin sections of leaf tissue.
Musetti et al. 2004 is the reference if you would like take a look
Dear Rita Musetti,
Thank you for you response. I will look into your manuscript.
Best Wishes,
João
hello Joao Paulo,
you can go through this article, they used H2O2 for ultrastructural detection where cerium perhydroxide was identified by TEM.
H2O2 localization in the green alga Micrasterias after salt and osmotic stress by TEM-coupled electron energy loss spectroscopy.
Dear Preeti Puskar,
Thank you for sharing this manuscript citation. It a very nice reference.
Cheers,
João
cerium chloride: doi:10.1093/jxb/ers277
I tested five years ago (OMG!) and it worked, it was pretty simple.
best,
Poli
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