Hello,

I might be misinterpreting the question. Are you asking the procedure of cloning a transcribed human gene into something like a plasmid? If this is the case, I can think of two ways. Regardless of the method, you will need to know the sequence of the gene you are working with. The sequence can be fetched from NCBI.

For the first method, you can reverse transcribe the total RNA with primers specific to the gene. Sometimes sequence of working primers can be found through literature. Or you can design the primer pair using a program like primer3. After reverse transcription, you can amplify the gene using PCR with the same primer pair. It can be difficult to clone the whole length gene with this method as one primer need to be at the beginning of the sequence and the other at the end of the sequence.

As an extension for the first method, you can clone the gene from DNA. This case you have the option of getting the full length gene by using primers that anneal to flanking regions or regions that are not on the gene. However, since you are using eukaryotic cells, such method will give you introns in the gene that you cloned. Introns are untranslated regions of the gene that are spliced out before proceeding to mature mRNA. For reverse transcription, I have used Omniscript RT kit from Qiagen. For PCR, there are many kits that you can use. I order my primers from Operon. You can get a primer pair in two days for about 30 bucks.

The simplest option, if you know the sequence of the whole gene, is having the gene synthesized by a company like Operon or GenScript. Using the synthesizing option, you can also optimize codons. This can be necessary if you are trying to express a human protein in a distant organism like E. coli.