Hello! I want to characterize Mycobacterium gordonae, which is a slow growing mycobacterium, scotocromogena. I have tried the PFGE technique following the protocol proposed by Iker Sevilla et al., in the article entitled: Pulsed-field gel electrophoresis profile homogeneity of Mycobacterium avium subsp. paratuberculosis isolates from cattle and heterogeneity of those from sheep and goats, with which I had the results shown in the attached image.
Article Pulsed-field gel electrophoresis profile homogeneity of Myco...
If someone who has worked with mycobacteria and electrophoresis in a pulsed field could help me out, suggest me an alternative where I can obtain a better PFGE's resut, or give me an explanation of what could possibly be the reason why I am obtaining this result (if happens that you have had this kind of problem), I would be very grateful for such information.
Dear Katherine, we use PFGE for staph and listeria. But the attached file can help you to increase the resolution of your PFGE gel. I think the cell amount is low. Also, addition of thiourea into running buffer may be usefull to prevent DNA degradation.
Best regards.
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