I prepared liposomes using the Thin Film Hydration method and encapsulated methylene blue. I reduced the liposome size to an average of 160 nanometers using sonication and extrusion. To remove free methylene blue, I employed Size Exclusion Chromatography. However, I collected the liposomes in a volume of approximately 30 mL of PBS, which is much larger than the target volume of 1 mL. I would like to reduce this 30 mL volume to 1 mL without disrupting the liposome structure, affecting the amount of liposomes, or altering the amount of methylene blue encapsulated within the liposomes. (NOTE: I currently do not have access to a centrifuge.)
I kindly request your assistance in addressing this matter.
You can use syringe microfilters to concentrate the nanoparticles and remove excess solvent. To retain your 160 nm liposomes while allowing PBS to pass through, select a filter with an appropriate pore size. Since 220 nm microfilters are commonly available, it may be worth trying to determine if they can effectively retain 160 nm nanoparticles. The selection of the pore size depends on the specific filter material and the size distribution of your liposomes.
- Badges
- Science topic
- Similar topics
- Mathematical Sciences
- Graphs