I encapsulated Vitamin D3 in a DPPC+cholesterol liposome (SUV). The [VD3] I put when creating the thin lipid layer was 0.75 mM. But, when I analysed my liposome system in HPLC, [VD3] detected in the system was 0.9 mM. How could this possibly happen? The increased [VD3]?
Dear Medina,
It is well-known that lipophilic molecules or lipid-soluble compounds such as vitamin D3 can be incorporated into liposomal bilayers by dissolving these molecules together with the lipids.
See also:
https://www.researchgate.net/profile/Sophia_Hatziantoniou/publication/23414561_Nanoliposomes_and_Their_Applications_in_Food_Nanotechnology/links/09e4150a9f63a7e25c000000/Nanoliposomes-and-Their-Applications-in-Food-Nanotechnology.pdf
In your case vitamin D3 (partly) incorporates in the lipid membrane (and/or binds to the surface) and basically ‘concentrates’ at the surface and in the bilayer. Overall the effect is that you end up with higher concentrations at and in the liposomes.
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