Hi there,
I am trying to set up my next experiment, but I am not confident I correctly understand the basic principle behind spontaneous immunoglobulin production.
How can PBMCs from healthy humans, when cultured in vitro (for example in 96-well plates) and without any stimulants, produce antibodies? Is it because of a cell subpopulation that is pre-activated? And according to the protocols, why does it take 7 days for cells to produce antibodies? Is it for B-cell differentiation to plasma cells? But once again, what is the differentiation trigger?
Could this be also the reason for the elevated basal expression of AICDA gene (5 fold via qPCR), that I found in my PBMCs, 72 h after the start of incubation? Are the B cells turning into plasma cells?
PBMCs were cultured in RPMI-1640, with only 5% charcoal-stripped FBS.
I would greatly appreciate your help and expertise and thank you in advance for your time,
Chris
PBMCs are so sensitive and during collection of BPMCs lots of factors need to be taken care. Sometimes vigorously pipetting, contaminated pipette tips..etc may activate the plasma cell receptors. Anyway cells are outside of their natural environment, so little disturbance may activate them to differentiate.
Hope it help you to find the reason.
- Badges
- Science topic
- Similar topics
- Mathematics
- Algebra
- Matrix