How to work out the final concentration of antibiotic on a resazurin-based assay?

A resazurin-based assay was produced with ampicillin against E.coli , negative control was just LB media and E.coli and the positive being ampicillin and E.coli, with resazurin for the readings....

16 December 2019 9,321 1 View

Does anyone have a recommendation for HA antibodies to use for IP with Dynabeads? If it's a rabbit antibody, do you use protein A or protein G?

Does anyone have a recommendation for HA antibodies to use for IP with Dynabeads? If it's a rabbit antibody, do you use protein A or protein G?

02 February 2016 5,517 2 View

Would SYBR Green dye interfere with pyro sequencing?

DNA was amplified using a PCR mix that included SYBR green dye. Could this DNA amplification undergo pyro sequencing without interference from the dye?

31 January 2016 9,563 4 View

Encapsulation efficiency of nanoparticles?

I have an question. How to calculate encapsulation efficiency of nanoparticles encapsulated in hydrogels?

19 January 2016 6,769 2 View

What is causing these Taqman probes with the same sequence but different dyes to perform differently?

We are having a perplexing issue with some IDT double-quenched probes. We have a particular amplicon with a SNP and have control DNA that is wild-type, heterozygous, and homozygous for the SNP....

10 January 2016 1,438 2 View

What are the alternate loci?

I am currently designing a SNP genotyping assay for CYP2D6 and I am confused about the alternate loci that keep appearing in my blast searches. What is an alternate locus? Does every gene have an...

12 March 2015 4,669 3 View

What does the allele CA|CA in the 1000 Genomes Browser mean?

13 January 2015 9,292 1 View

How do we guide carbon cycle science research to increase its usefulness for policy makers?

16 April 2013 9,939 4 View

Internships for PhD interested in medical & biological imaging

I hope this is a good place to post my question! I am currently a PhD student, and as part of my program I have funding available to complete a short (~ 3 months) placement at the end of my PhD...

01 January 1970 2,685 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View