How can you investigate a cis/trans isomer with COSY NMR?

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Teodoro S Kaufman

You do not need COSY if your signals are unequivocal. Cis isomer has smaller constant coupling tan trans congener.

Daniel Tietze

If you are not restricted to a COSY and if your are investigating peptides or proteins, just measure a 1H-1H TOCSY. The cis and trans isomers conected to Xaa-Pro isomerism are easy to identify.

Milco Lipreri

The distances in the space between the hydrogens cis/trans and adjacent hydrogens are different so you can also use NOESY experiment.