Which is the appropriate statistic test to examine the relation between students' affective state and academic performance?

Which test should I use to compare students' mood effects on academic performance? I want to compare mood measured on a likert scale (from point 1= very sad to point= 5 very happy) to three...

04 February 2021 1,907 5 View

I am trying to form microspheres with PCL and PVA using a mechanical stirrer but the polymers keeps forming clumps. What do i do to disperse them?

I prepared a 1% PVA, and dissolved 500 mg of PCL in 5 ml of DCM. Then I introduced the PCL solution into PVA while stirring at 2000 rpm for 2 hours, after which i place it on magnetic stirrer to...

15 April 2019 7,177 4 View

Can normal colon cell lines form spheroids?

I am growing spheroids from HT29 colon cancer cells, and I need some sort of control cells to be compared with the HT29. Now, I should grow the control cell into spheroids and then use these new...

30 October 2018 3,719 12 View

Please, can I get a research paper or an article on lipid extraction from ogbono and egusi using chloroform-methanol method ?

I am working on a project that involves lipid extraction using chloroform-methanol as solvents. I washed the product with water-methanol and about to use a rotary evaporator for evaporation. But...

10 May 2018 4,089 3 View

What is the effect of differentating 3T3-L1 preadipocytes in differentiation cocktail for 3 versus 7 days?

I noticed that some protocols use differentiation media for 2-3 days while others go for 5-7 days before changing to maintenance media. Why is that? Is an adipocyte grown in DM 3 days + MM 4 days...

08 December 2017 1,792 1 View

How to calculate sample size for a moderation study?

Hi, I am doing a study design and planning to do a moderation study. This is the simplest model. However, I am struggling with the calculation of sample size. How do I calculate the sample size...

23 April 2017 5,449 8 View

I want to take for the first time CHO cells in culture. How can I get 100% sterile glass Erlenmeyer flasks to use them again in the cell culture?

29 November 2015 9,661 3 View

How do I design primers that will distinguish between highly homologous transcripts?

10 August 2014 4,948 4 View

How can I get my ligation to work?

I've been attempting to subclone an insert from one vector to another by PCR amplification with primers that incorporate XhoI/EcoRI sites at the ends of the insert and then digesting the insert...

30 July 2014 4,230 6 View

Does anyone know which markers to use to identify human regulatory Macrophages (M2b)?

I know that for murine M2b SPHK1, LIGHT and Arginase1 heve been described as markers, but I cannot find other markers for human M2b except the IL-10 high and Il-12 low phenotype.

12 November 2013 5,762 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Why Trivalent chromium start precipitating above PH 5 ? Moreover discuss difference between adsorption of chromium and precipitation of chromium ?

I am a student of MS Environmental Engineering and started my research phase. I am working on metal removal ( chromium iii ) by using activated carbon prepared from the agricultural feedstock. So...

01 March 2021 5,475 2 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View