How can we sequence a specific chromosomes (or part of it)?

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Abhijeet Singh

There are several methods to isolate chromosomes and further processing, and sequencing by different sequencing technologies.

But I was wondering what can be the size of the genome you are working and which organism is it? If the region of interest is >7 Gb, which is more than several whole genomes. I can't even imagine the size of complete chromosome and whole genome.

Assa Yeroslaviz

This is embarrassing. Sorry about that, but I was off by 10x.

I meant 7Mb part of the chromosome. It is the mouse genome BTW.

Ali Javadmanesh

Hi Assa

You need to talk to an NGS company for consultation about designing a specific panel of sequencing for the desired region. If you have a reasonable number of samples, it would be cheaper than whole genome sequencing.