Greets!! Tou can use Colum chromatography, by using sovlent system start with highest nonpolar solvent then increase polarity gradually, by addition of methanol, like you can start 100% chloroform then start increase polarity by increase % of methanol as 5%,10%,20,30,40,50,,, untile the column silica be clear, each fraction should be collected and test its NMR and Mass spectroscopy, but first you should develop a good TLC plats can detects the diffrents spots of extract