Try to place the specimen in a drop of hot Gelatin, on a clean glass slides under a suitable power of a binocular stereoscopic microscope and cutting transversely by a sharp razor with the help of a needle

thank you for your answer Massakib Bekkaye.

Hello.

We have recently published a boo chapter on guard cell isolation- very extensive and easy to follow.

Zhu, M., Jeon, B. W., Geng, S., Yu, Y., Balmant, K., Chen, S., & Assmann, S. M. (2015). Preparation of Epidermal Peels and Guard Cell Protoplasts for Cellular, Electrophysiological, and-Omics Assays of Guard Cell Function. Methods in molecular biology (Clifton, NJ), 1363, 89-121.

Link:http://download.springer.com/static/pdf/118/chp%253A10.1007%252F978-1-4939-3115-6_9.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Fprotocol%2F10.1007%2F978-1-4939-3115-6_9&token2=exp=1449164544~acl=%2Fstatic%2Fpdf%2F118%2Fchp%25253A10.1007%25252F978-1-4939-3115-6_9.pdf%3ForiginUrl%3Dhttp%253A%252F%252Flink.springer.com%252Fprotocol%252F10.1007%252F978-1-4939-3115-6_9*~hmac=ea19cba29c7d5406f5b601ed0123fd23760d3843578a182e8f48b1170fe84d69

Thanks,

Biswa

Hi Biswa,

Thanks a lot for your answer. We have developped since that time a good technique with the imprits method, but I'll for sur read your paper. Thanks again Biswa.

Safia

Good question. Following to learn ...