I want to know if I can identify a circulating tumor cell which is shed from a primary tumor. How can I be specific that the cell which is identified as CK+ve/CD45+ve is a cell which is shed from tumorous liver?
Are ASGPR and Glypican the best liver-specific markers?
You are correct Patricia. Coz this CTCs are non hematopoietic, so obviously it must be CD45-ve cells.
Thank you for your time.
cytokeratin 8, 18,19 and AFP are good marker for HCC CTC (that are cd45 negative) and in particular for HCC but you have to permeabilize cells to detect them. Epcam is usually restricted to stem cells in HCC then it may be not the best marker in this cancer type. You can also use CD73 that is expressed on hepatocytes (but also on CD45+ cells). ASGPR should be the most specific marker but in my hands it do not work properly.
Thank you so very much Federico,
It sounds great. I feel you have a good experience on HCC/CTC.
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