How can we isolate RNA from the dry seeds of Brassicaceae plants with oil content?
Agree with Vinay, Trizol may work. Give Invitrogen tech support a call/e-mail, they are very helpful.
I think you should try TRI reagent. It helped me isolate RNA from samples preserved in methanol for over a week. Good Luck.
I agreed to answer with all of use of TRI reagent but our previous experience with phenolic rich plant and oil seed show that conventional Lithium chloride method also give good significant RNA with purity of 1.75-1.83....good luck
With phenolic rich and polysaccharide rich plants (plant tissues) i would recommend CTAB method.. A good protocol working for me with polysaccharide rich elm leaves is this... http://www.ncbi.nlm.nih.gov/pubmed/17952668
Just change the isopropanol precipitation step at the end with LiCl.. You will get lots of RNA
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