Use of herbicide in crop production is a vital management technique to get higher productivity and low cost of cultivation. As a result, its indirectly add some quantity of chemical element in the environment. for example, chlorinated herbicides are releasing more amount of chlorine element. The herbicide residues can be estimated by using HPLC and GC. The protocol using to estimate the residue is expressing the quantity which has been more than Below Detectable Level (BDL). But, still the herbicide residues are persistent in the environment. Some group of herbicides adds more residues to the field. It’s more important to study the effect of herbicides on the environment.