How can we disperse salt particles in the polymer solution for preparing polymeric scaffolds by solvent casting and particulate leaching method?

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Do High or low A260/A230 and 260/280 ratio for isolated RNA make problems in real time PCR results?

I extracted RNA from the animal tissues by Trizol and phenol technique. The A260/A230 and 260/280 ratio were sometime lower or higher than a normal range. Do this make problems for my real-time...

21 October 2023 1,042 3 View

How I can design primer for promoter region for real time PCR?

I know its specific location.

16 June 2023 6,889 7 View

How can I analyses a split split plot design by SPSS?

I have a split split plot design with a main factor(4 levels) and a subfactor (2 levels) and a sub sub factor (2 levels) with 3 replications: 1-How should I define the method on SPSS? 2- for post...

16 January 2023 5,688 8 View

Who can help me about extracting data methods and analyzing from literatures?

I have some questions about data extraction methods from soil-environmental literature.

03 February 2022 9,744 2 View

Reaction kinetics of light naphtha isomerization with pt base on chlorinated gamma alumina?

we need the reaction kinetics for light naphtha isomerization when catalyst pt base on chlorinated gamma alumina is used.please guide us, in which book or article we can find it.

23 October 2021 7,547 3 View

Have you experienced multiple case studies in qualitative research?

I'm going to begin research with about 100 participants of different ages in qualitative research. Using multiple case studies for drama therapy is my aim. Is it possible to have multiple case...

29 July 2021 6,134 9 View

Can anybody help me identify the disease (and the related treatments) in adult lates calcarifer (cage culture)?

The symptoms: Infected fish with bloody liver; abdominal adhesions; exanthema; scale loss; body (skeletal), opercula and eye deformity; fin rot, mouth and skin ulcers, ascites, and blindness.

15 January 2020 9,429 6 View

What are differences between geothermal and hydrothermal systems?

04 January 2019 699 13 View

How can I determine the protonation state of a phosphorene sheet in acidic and neutral pH conditions?

Dear experts, I am going to perform an MD simulation on a system including water and phosphorene sheet in different pH conditions (low and neutral pH). However, I do not know if the different pHs...

30 December 2018 9,651 1 View

Is there any way to produce the same random numbers in R when using Linux or Windows?

I'm getting slightly different random numbers depending on the OS (Windows vs Linux) although I have specified the seed using set.seed. Is there anyway to guarantee reproducibility across...

12 March 2018 9,978 10 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

Who will be moral responsible for the death of thousands of people in the event of an earthquake?

Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...

08 August 2024 6,134 12 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended?

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...

05 August 2024 8,056 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Regarding a model for simulating battery charge and discharge, what do you consider to be high fidelity?

Regarding a model for simulating battery charge and discharge, what do you consider to be high fidelity? What is the acceptable percentage of error (regardless of the metric)? Could you suggest...

03 August 2024 5,358 0 View