Plants usually uptake nitrogen in the form of ammonium and nitrate. If we supply 15N labelled nitrogen fertilizer in the form of ammonium which will eventually get nitrified. I am interested to know how can we differentiate how much ammonium and nitrate were consumed by plants. I will be thankful for any suggestion, guidance, advice or relevant literature.
@ Sajjad, Plants preferred NO3 - in the drier environments and NH4 + in the wetter environments. NO3- is main form of nitrogen taken up by upland crops while NH4+ is prevalent form taken up by plant grown under submerged condition. Nitrate is the form of nitrogen most used by plants for growth and development. Nitrate is the form that can most easily be lost to groundwater. Ammonium taken in by plants is used directly in proteins. This form is not lost as easily from the soil.
If you want to preserve ammonia in that form you need either sterile conditions or to add a nitrification inhibitor. Within limits, internal ammonia concentrations are controlled to avoid toxicity. Uptake of ammonia instead of nitrate also impacts on H+ excretion and cation uptake.
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Nice response Paul Milham. You mean, ammonium uptake is associated with more uptake of other cations than nitrate..??
Nice response Paul Milham. You mean, ammonium uptake is associated with more uptake of other cations than nitrate..??
Plants those love to take nitrogen in aerobic condition they uptake nitrogen as ammonium, generally maximum vegetables uptake their nitrogen as nitrate form. Plants those growing in submersible /anaerobic condition as rice love to take up nitrogen as nitrate.
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