You can not. The only available test for this purpose is coagulase test.

Other tests or agar media are unreliable.

Regards

Dear Pradeep,

Mannitol salt agar based selection is for salt tolerant organism, most of the staphylococci species are salt tolerant including CoNS. Mannitol fermentation will differentiate the S. aureus from mannitol non fermentor epidermidis and other CoNS.

Ultimate biochemical test to differentiate CoPS and CoNS are DNase and coagulase.

Best

Hi

P agar supplemented with acriflavin.

Which can be used as a confirmation test of the beta-galactosidase.

Hello, several medias such as:

1. Baird-Parker agar

2. Calf-blood agar

3.Vogel-Johnson agar

4.Carter agar

Hope this info will help you!

I suggest blood agar plates test. coagulase positive bacteria formed a yellow colour colony whereas, coagulase negative bacteria (S.epidermidis) formed a white coloured colony.

Mannitol salt agar is the best media for differentiation of CoNS and CPS in which the letter ferment mannitol( golden yellow)and the former(pink) does not plus to to this hemolysis on sheep blood agar.

Baird-Parker agar (BPA) is a good alternative for differentiation between CoNS and CoPS with the ability also of confirming the presence of Staphylococcus aureus. CoPS grow on BPA as black or grey colonies.

If BPA is supplemented with egg-yolk (see also ISO 6888-1:1999), CoPS colonies are surrounded by a clear zone and after incubation for at least 24 h an opalescent ring, immediately in contact with the colonies, may appear in this clear zone. If BPA is supplemented with rabbit plasma fibrinogen (RPF) (see also ISO 6888-2:1999), small colonies surrounded by a halo of precipitation, indicate coagulase activity. BPA with egg-yolk requires further confirmation of the colonies isolated on agar (i.e. coagulase test), while BPA with RPF does not require a confirmation step since RPF is based on coagulase reaction.

Yolk salt agar is another option for differentiation between CoNS and CoPS.

You can not. The only available test for this purpose is coagulase test.

Other tests or agar media are unreliable.

Regards

Thank you all for your reply. I will try Baird Parker Agar supplemented with rabbit plasma fibrinogen. I may be able to differentiate CoPS from CoNS. Special thanks to @ Nikolaos D Andritsos for your suggestion.

Baird Parker media

Dnase media

Coagulase test

One of the method for differentiation is proposed by Kloos and Schleifer (1975) and Bannerman (2003) which is a set of biochemical test.

Hello!

You can differentiate between S. aureus(coagulase positive) on Manitol salt agar this species on it appear yellow because can ferment Manitol while Cons can't ferment this sugar and appear white(S. epidermidis)

Hello ,

I agree with Prof . Alaa Hani Al-Charrakh .

You can use Baird Parker Agar (with rabbit plasma fibrinogen ) or Mannitol Salt agar, but you should confirm the results with tube coagulase test . For CoPS you should get really dense and firm clot (4+ reaction). From our experience, the 4+ reaction was always in correlation with production of other important enzyme of St. aureus - thermonuclease, but 3+ reaction sometimes gives negative results.

Attached you can find the procedure and interpretation of Tube Coagulase Test.

Beta hemolysis on blood agar

confirmation can only be the coagulase test

Mannitol salt agar

Beta hemolysis on blood agar

coagulase test

I can guess from yellow pigment and beta-haemolysis of S.aureus but it is only presumptive. The most special feature that I utilize is microscopic appearance of Gram-stained colony of S.aureus or coagulase negative staphylococci. Generally the first thing I do is Gram Stain, then coagulase test, because microscopic appearance of S.aureus on Gram Stain is very different from the most coagulase negative staphylococci .

mannitol salt agar., mostly s.aureus produce the yellow pigment in NA, and beta hemolysis.

The best method is coagulase test (slide and tube methods).

The best option is to use Chapman Agar (Manitol-salt) to differentiate CoPS and CoNS. There ara other options to differentiate between spieces that are CoNS, for example antibiotics resistance.

The reliable test is Coagulase test, but you can appreciate colony characteristics on Manitol Salt Agar

I am studied in this subject, my thesis was in the isolation of Coagulase - positive and negative Staphylococci.

You can use Vogal – Johnson medium and Baird – Parker medium to differentiate the Coagulase - positive Staphylococci from Coagulase - negative Staphylococci and Purple agar to distinguish between Coagulase - positive Staphylococci.

I think to be use PCR techniqe for quickly differentiate between the positive and negetive coagulaes of Staphylococcus bacteria.

Follow

In addition to MSA you can use Dnase agar

Beta hemolysis on blood agar

coagulase test

Dnase agar

PCR techniqe

1.culture on mannitol salt agar

2.coagulase test

3.hemolysis on blood agar