Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Best rat model of Type 2 diabetes and metabolic syndrome?

Hi Im seeking advice on what would be the best model of T2D and metabolic syndrome in rats Thank you

04 September 2020 3,661 4 View

How can I digest the brain tissue in healthy mice as evaluating the apoptosis using flow cytometery by annexin V/propidium iodide (PI)?

We digested the brain tissue with Trypsin ( 1x) for 15 min (all steps were performed in cold conditions) and suspend the cells by pipetting up and down (3-4 times) to the single-cell suspension....

22 August 2020 5,611 4 View

Hi, is there anyone tell me or share me a link for downloading the template for "Cities" journal of Elsevier?

I went through the cities journal website but unluckily couldn't find the template

28 June 2020 811 3 View

Can anyone suggest a kit or protocol to extract Brucella spp. DNA from FFPE blocks?

Hi everyone! I am trying to extract Brucella DNA from FFPE blocks. Can anyone suggest me any good kit or protocol? I checked many articles and kits but still could not find an appropriate kit or...

15 December 2019 9,767 2 View

Photovoltaics are solar energy, renewable and economical. Why are people not very attracted by the benefits of domestic use of this green energy ?

Hello, Photovoltaics are solar energy, renewable and economical. Why are people not very attracted by the benefits of domestic use of this green energy ? Thank you

06 April 2019 8,782 19 View

What is the contribution of renewable energies in the fight against global warming?

The phenomenon of the greenhouse effect is a threat to the environment. What is the contribution of renewable energies in the fight against global warming?

04 April 2019 1,239 33 View

Methodology for implementing a thesis ?

Methodology for implementing a thesis involves: - State of the art of the studied phenomenon - Study context - Introduction - Hypotheses - Objectives - Labor interest - Methodology apprehended -...

14 February 2019 6,502 16 View

The passage from micro to nano scale in indentation of materials is of concern to researchers. How does one proceed to arrive at relevant answers?

The passage from micro to nano scale in indentation of materials is of concern to researchers. How does one proceed to arrive at relevant answers?

24 January 2019 5,584 2 View

How is 3D pelvic kinematics examined during walking before and after interventional exercises in patients with pelvic inclination?

in general, for healthy people, in the 3D motion analysis to define the anatomical coordinate system on segments, one frame in static position is taken by the camera, which will make segment...

10 October 2018 4,098 2 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

How to discretize a continuous time state-space model in MATLAB correctly?

I am trying to discretize a continuous time state space model using the following code s=tf('s'); G=1/(Iyy*(s^2)) Gs=ss(G) Gd=c2d(Gs,0.01,'zoh'); Now, when i use this discretized model 'Discrete...

30 January 2021 8,665 10 View

SAR X-Band constellation from Capella Space?

Dear all, I'd like to open here a sort of forum for understanding how the geodesists community is moving in view of the X-band SAR satellite constellation. The new constellation will offer new...

24 January 2021 6,248 0 View

What's the effect of COVID-19 pandemic on perception of open green areas?

People long for green space Anxiety, worry Restriction of recreational activities What do people think of green spaces because of Covid 19 pandemic? With which modeling this issue can be studied

28 December 2020 5,243 3 View

How can the amount of accumulated dust be estimated?

Hi Kind helpers, For my industrial design thesis, I'm working on a concentrated solar energy (>CPV) construction, to be integrated in urban areas/buildings (glass roofs, with integrated...

28 December 2020 9,180 8 View

How do I linearize a model that contains the min() function, to derive state space matrices?

I'm trying to linearize a non-linear model and write it in state-space form (derive state-space matrices) for control and optimization purposes. The nonlinear model is: x(k+1)=f(x(k),u(k),d(k)),...

06 December 2020 9,029 3 View

How is the controllability, observability and invertibility of a system determine ?

How is to determine the controllability and observability of a system in frequency domain or in Laplace domain? Usually these two important factors are described by the rank of the system...

02 December 2020 3,919 8 View

Do you know any country-specific methodologies of urban public spaces evaluation?

Hello! Currently I am working on a research about different methodologies of urban public spaces evaluation/their impact assessment. The only comprehensive projects with explicit measurement...

18 November 2020 2,428 5 View

What is the Timestep definition in the Conservation Element / Solution Element in 3D ?

15 November 2020 1,214 3 View