How can be computing of PI from this sequence (Pro-Cys-Arg-Val-Glu-Arg)
And what is the net charge at pH 14
Hi Atheer,
You can calculate a therotical pI and Mw from ExPASy software.
Here is your result,
Theoretical pI/Mw (average) for the user-entered sequence:
PCRVER
Theoretical pI/Mw: 8.68 / 758.89.
There is a peptide calculator in the form of an excel sheet here,
spdbv.vital-it.ch/TheMolecularLevel/Goodies/PeptChg.xls
This allows you to put in the number of each amino acid in third column on left and the graph will autocorrect net charges for your particular peptide. I presume this is an internally sequenced fragment so place 0 at N and C terminus values and type in the number of each amino acid as above.
Hope this helps,
Katrina.
At http://pepcalc.com/ you can find MW = 758,89 (as given by Katarina) and pI = 9.29;
The calculator can also present charge to pH plot and hydropathy plot. I use it very often. There is also one more that I could recommend:
http://www.bachem.com/service-support/peptide-calculator/
From the charge to pH plot it comes that at pH 14 the charge is -3.
Hi!
Here is the link for the Expasy website:
http://web.expasy.org/compute_pi/
Thank you Prof Dr. Yasset Perez for this kindly package and papers ... cheers
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