Very interesting and educational question, for work in experimental studies with animals! I would like to follow the discussion, which I suppose can be very useful to me and my work!
Thank you for raising such a useful question. A good piece of work: Ahn, S. J., Hong, H. K., Na, Y. M., Park, S. J., Ahn, J., Oh, J., et al. Use of Rabbit Eyes in Pharmacokinetic Studies of Intraocular Drugs. J. Vis. Exp. (113), e53878, doi:10.3791/53878 (2016).
The typical clinical drop volume is 30-40 microlitre and this volume is used in non-clinical studies with rabbits that dispenses the formulation with the intended clinical dropper bottle. But the human palpaebral fissure can retain upto 25 microlitre and the excess flows out or drains into the NLD. A topical low dose test volume of 10 microlitre is recommended as this accounts for the rabbit's smaller eye size relative to the humans and provides better correlation. The ocular dosing frequency is adjusted at QID in rabbits for BID dosing in clinical subjects. This is done to provide uniform exposure to the test drug when physicochemical factors serve as the confounders in the resultant drug concentration.
Thanks Ranjita Santra for your valuable guidelines regarding ocular dose of a test drug for rabbits.
I would like to focus on further discussion in this forum dear RG members.
My basic question was, if an oral dose/kg body weight (human) of a drug is known, then how can we calculate ocular dose for a test animal like rabbit? is there any formula to calculate?
Dear Ranjita, Please share the reference if any you have regarding "the low dose test volume of 10 microlitre is recommended as this accounts for the rabbit's smaller eye size relative to the humans and provides better correlation".
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