What could be source of contamination for seeing positive amplification in negative control?

Yesterday, I ran a gradient endpoint PCR with some primers and saw positive amplification for positive and negative for negative amplification. Running the same assay today, show positive...

13 January 2021 5,201 1 View

I have a monoclonal antibodies (purified) of 8mg/ml, Now I want to evaluate ELISA for my serum samples in International ELISA UNITS?

I need to know how to convert micrograms of antibodies into ELISA Units.

21 September 2020 3,286 3 View

Has anybody found better anticancer effect on patient derived primary cancer cells than cell line?

I am testing compounds on the patient's primary cancer cells as well as a cell line. Generally, I see better activity in cell lines compared to primary cells. Surprisingly I am seeing the...

18 March 2020 467 3 View

Is there an alternate for UV Ozone treatment in order to pursue ETL surface modification?

I wish to deposit a perovskite layer over ETL (SnO2) in order to fabricate the n-i-p planar device and could notice the role of UVO treatment prior to deposition. I was wondering if there any...

28 January 2020 3,310 2 View

Is Girvan Newman and Louvain an effective technique to identify rumors in social networks?

Suggest methods to perform clustering and classification in these- 1) Graph structures 2) Twitter networks 3) Rumor Identification 4) Big data networks

03 December 2019 7,452 3 View

What are the factors affecting Wind Energy Production in Denmark?

Hi everyone, I am a Master's student and I am doing my thesis on Wind Energy Production forecast for Denmark and for that I need the data of the Factors affecting the Wind Energy Production in...

06 November 2019 7,535 2 View

What would be the formula for finding the stretch force for expanding an O ring made of elastic ? On what factors the expansion stretch force depends?

I want to expand an O ring made of elastic material and am looking for the mathematical expression (dynamics) on which the stretch force depends. In addition to this, how does it vary in different...

18 March 2019 4,158 3 View

Catalyst and electrode for fuel cell application?

Hello, could anybody help me to get idea about which kind of ionomers, surfactants, additives and solvents except Nafion, iso-propanol, n-propanol, hexanol, methanol, ethanol, water, ePTFE,...

11 November 2018 8,886 7 View

Is there literature on knowledge management in policy innovations?

I am looking for theories/ frameworks on how knowledge is created and managed in a network on the way to an innovation in public services.

22 September 2018 1,624 4 View

How is inter-coder reliability calculated in qualitative research calculated?

If we are doing a Grounded Theory research involving coding of interview transcripts as propounded by Glaser (1978) and Strauss and Corbin (1990), then: 1. Is there a heuristic to decide on the...

23 October 2017 6,494 5 View

Can I run spearman's correlation on non monotonic data? Is it advisable?

Hi all, I am running a partial correlation analysis on mood data. I want to see if there is a correlation between scores on a novel hypomanic mood scale and existing measures of hypomania - the...

25 February 2021 1,619 3 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

Interpretation of Goodman - Kruskal Gamma squared?

Dear colleages, Eta squared is dominantly used in reflecting the explaining power the same manner as a squared partial correlation coefficient (R2p ) from multiple linear regression: as the...

22 February 2021 7,801 3 View

How do I group questions of SPSS under one variable for Spearman's r?

12 February 2021 5,379 5 View

What effect size should i use for a meta-analysis when study data violate normality assumptions?

I'm looking for advice regarding what effect size to use for meta-analysis. Our question is whether the number of individuals in a group affects the per-capita productivity of that group. Does...

10 February 2021 5,510 8 View

Recommendations on CO2 Resistant Rocking Shaker?

A certain protocol we have developed requires the use of a Rocker/Rocking Shaker in which our dishes with cells are maintained in the incubator. The rocking is essential to the protocol but we...

09 February 2021 3,291 1 View

How to calculate cross correlation of two signals from the same source one of which is going through a filter ?

A signal is split into two parts and one of them is going through a filter (say, with a transfer function H(f)) and the other part stays unchanged. Then I want to know how to calculate their cross...

01 February 2021 5,101 8 View

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29 January 2021 2,623 1 View

Why is Kendall's Tau always so high?

Hi there, I am trying to estimate the agreement of judges on the ranking of certain individuals - in particular, the agreement of all other judges with the first judge. I was thinking to use...

28 January 2021 3,121 4 View

Similarity between these two waveform? Similarity in terms of Shape?

Hi, These are the outputs generated by a system. Green-colored (nr) is desired output, while the system generates Red-colored (nf). I also have a dataset in CSV file. For this waveform, What...

21 January 2021 929 2 View