Hi Vladimíra! How are you??

Are you having trouble with the hplc detection conditions or the sample pre-prep ? Or both??

This paper may not be in english, but if it interests you by looking at the abstract, I may help translating it!

"Analytic methodology validation for thymol dosages in vegetable extracts of Lippia sidoides Cham by HPLC" 

RogÈria de Sousa Nunes;; Bety Anne de Albuquerque Senna; JosÈ Alexsandro da Silva & Davi Pereira de Santana.

Thank you very much for your help.

I will study these papers.

Thank you. 

see: http://www.sigmaaldrich.com/catalog/product/sigma/g4259

G4259 SIGMA β-Glucuronidase from Helix aspersa (garden snail) can be used to convert glucuronide to alcohol. If you use this method, you analyse the sample before and after enzymatic conversion and the change in alcohol indicates the concentration of glucuronide. 

Thank you very much Thomas, we have ordered this enzyme :)

Hi Vladimira

liver tissue store secondary metabolites (as antioxidants) soluble in fat (lipophylic) as vitamin A D E K, I think not all secondary metabolites 

Hello Eshak

Do you have some papers related to this topic, please?

There is another product from a different snail with both sulfatase and glucuronidase activity:

http://www.sigmaaldrich.com/catalog/product/sigma/g0876

http://www.perkinelmer.com/catalog/product/id/nee154001ea

Glusulase is the older name used by Perkin Elmer.

Hi Vladimira,

If I had to do this type of analysis I would develop an UPLC-MS (QTOF or similar MS) method to detect your intact compounds of interest. The method must be able to detect very low levels of the compounds and you would need to work on a good sample preparation procedure to selectively extract and concentrate your samples up.

Using metabolomic software (eg. MetaboLynx from Waters) you can monitor the metabolomic transformation of your compounds. Using this approach you can also monitor expected and unexpected metabolomic transformations.