We want to analyse thymol, carvacrol and their metabolites from liver tissue.
First you need to prepare calibration curve from thymol, carvacrol and their metabolites standards. You need to optimiza an HPLC method for those metabolites using trial and error experiments by trying different mobile phases like methanol:water, Acetonitrile:water:formic acid and nonpolar HPLC column; C18 for example, it is useful for plant monoterpenes.
Agree with Detlef. Since the metabolites you are studying are volatile compounds and it is very easy to optimize a method of analysis on GC-MS.
Thank you very much for your answers. We know, that liquid chromatography is sensitive method to identify plant secondary metabolites from plasma or urine (attached papers). We focus to metabolism of plant monoterpene thymol in the liver. Thymol is probably metabolized to thymol-sulphate and thymol-glucuronide in the liver. Liver tissue is a complex matrix and we don´t have experiences with preparation and analysis of this kind of sample by using LC.
I know that thymol as plant compound is volatile. I´m not sure whether thymol-sulphate and thymol-glucuronide are volatile or not.
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